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目的研究如何运用随机扩增多态DNA(RAPD)技术判定鼠类种属及来源。方法取鼠尾组织制备DNA基因组,并进行RAPD扩增,以强带差异来区分鼠类遗传多态性。结果建立了褐家鼠DNA快速制备方法,并获得基因图谱。根据120条引物各自表现出的不同多态性,合成一组褐家鼠的通用引物,定名为P1(5′-TCG GCG GTT C-3′)、P2(5′-GAGAGC CGT C-3′)、P3(5′-ATG GCA TCT C-3′)和P4(5′-CTT GGC ACG A-3′),结果显示,该组引物对褐家鼠在目的片段(925 bp)处均有良好的扩增。结论分析个体DNA序列同源程度判断遗传距离大小,可鉴别鼠的来源是否为输入性鼠类,对追溯某种鼠类扩散途径和历史以及预警鼠传疾病提供科学依据。
Objective To study how to determine the species and origin of murine by using random amplified polymorphic DNA (RAPD) technique. Methods DNA genomic DNA was prepared from rat tail tissue and amplified by RAPD. Differences of strong bands were used to distinguish mouse genetic polymorphisms. Results A rapid DNA preparation method for Rattus norvegicus was established and a genetic map was obtained. According to the different polymorphism of each of the 120 primers, a group of Rattus norvegicus universal primers were designed and named P1 (5’-TCG GCG GTT C-3 ’), P2 (5’-GAGAGC CGT C-3’ ), P3 (5’-ATG GCA TCT C-3 ’) and P4 (5’-CTT GGC ACG A-3’). Good amplification. Conclusion The analysis of the degree of homology of individual DNA sequence to determine the genetic distance size can be identified from the origin of the mouse is an importation of rodents, to trace a certain route of proliferation and history of mice and early warning rodent disease provide a scientific basis.