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目的探讨核因子-κB(NF-κB)及血清降钙素原(PCT)与新生儿败血症的关系。方法选择我科2008年10月至2010年10月住院的败血症新生儿为败血症组,血培养阴性、确诊感染的病例为普通感染组,同期收住的非感染性疾病新生儿20例为对照组。败血症组患儿分别于入院时和入院后7天,普通感染组和对照组于入院时采集静脉血检测外周血单个核细胞(PBMC)中NF-κB表达和血清PCT水平,所得数值进行组间比较。结果败血症组(29例)NF-κB表达及PCT水平在入院时明显高于入院后7天、普通感染组(26例)及对照组[NF-κB:(19.1±2.4)%比(6.9±2.0)%、(12.7±2.7)%、(4.8±2.1)%,PCT:(20.0±3.0)ng/ml比(1.8±2.1)ng/ml、(11.8±4.1)ng/ml、1.7±0.7)ng/ml,P均<0.05];普通感染组高于对照组和败血症组入院后7天,差异有统计学意义(P均<0.05);对照组与败血症组入院后7天比较差异无统计学意义(P>0.05)。结论 NF-κB和PCT是早期诊断新生儿败血症较好的实验室指标,动态监测其变化可以判断临床疗效。
Objective To investigate the relationship between nuclear factor-κB (NF-κB) and serum procalcitonin (PCT) and neonatal sepsis. Methods The neonates with sepsis were hospitalized in our department from October 2008 to October 2010 as sepsis group. The blood culture was negative. The confirmed cases of infection were normal infection group. Twenty newborn infants with non-infectious disease admitted in the same period were the control group . Serum levels of PCT and NF-κB were measured in peripheral blood mononuclear cells (PBMCs) from sepsis patients at admission and 7 days after admission, respectively. Venous blood was collected from common infection group and control group at admission, Compare Results The expression of NF-κB and PCT in sepsis group were significantly higher than those in control group (n = 26) and control group [NF-κB: (19.1 ± 2.4)% vs 6.9 ± (20 ± 3.0) ng / ml (1.8 ± 2.1) ng / ml, (11.8 ± 4.1) ng / ml and 1.7 ± 0.7 ) ng / ml, all P <0.05]. Compared with the control group and the sepsis group, the difference was statistically significant (P <0.05). The differences between the control group and the sepsis group were not significant at 7 days after admission Statistical significance (P> 0.05). Conclusion NF-κB and PCT are good laboratory indexes for the early diagnosis of neonatal sepsis. To monitor the changes dynamically can determine the clinical efficacy.