论文部分内容阅读
目的研究神经生长因子(NGF)对成年兔视神经夹伤后修复的影响。方法16只成年兔随机分成NGF组和对照组,每组8只兔。建立兔右眼视神经夹伤模型后分别将载有0.06mlNGF(浓度:5×10-4g/L,NGF组)或等量磷酸盐缓冲液(PBS)(对照组)的组织工程化神经移植于视神经损伤处;并向右眼玻璃体腔内注入0.02mlNGF(浓度:5×10-4g/L,NGF组)或等量PBS(对照组)。所有兔左眼为正常空白对照组。分别于夹伤后1d、2周、8周进行闪光视觉诱发电位(FVEP)检查。夹伤后8周时作光学显微镜和电子显微镜检查观察视网膜神经节细胞(RGC)和视神经的改变,同时用计算机图像处理系统作视神经纤维计数。结果夹伤后2周时FVEP检查结果显示,NGF组伤眼与健眼FVEP幅值比为0.765±0.150,对照组为0.494±0.108,NGF组与对照组相比差异有统计学意义(P<0.01)。夹伤后8周时NGF组伤眼与健眼FVEP幅值比为0.581±0.138,对照组为0.409±0.119,NGF组与对照组相比差异有统计学意义(P<0.05)。夹伤后8周时的光学显微镜和电子显微镜检查结果显示:NGF组RGC、视神经纤维的退变较对照组轻。夹伤后8周时NGF组和对照组视神经纤维计数分别为(10955±608.7)、(7898±608.8)根/mm2,两组间差异有统计学意义(P<0.001)。结论NGF能够在一定程度上增加RGC的存活,促进轴突的再生,因而对视神经夹伤后的修复、视功能的恢复具有一定的促进作用。
Objective To investigate the effect of nerve growth factor (NGF) on the repair of optic nerve crush injury in adult rabbits. Methods Twenty-six adult rabbits were randomly divided into NGF group and control group, with 8 rabbits in each group. Tissue-engineered nerves containing 0.06 ml of NGF (concentration: 5 × 10 -4 g / L, NGF group) or equal amount of phosphate buffered saline (PBS) (control group) Optic nerve injury; and into the right eye intravitreal injection of 0.02mlNGF (concentration: 5 × 10-4g / L, NGF group) or equal amount of PBS (control group). The left eye of all rabbits was normal blank control group. Flash Visual Evoked Potentials (FVEP) were performed at 1, 2 and 8 weeks after injury. Eight weeks after the injury, the changes of retinal ganglion cells (RGCs) and optic nerves were observed under light microscope and electron microscope. Meanwhile, the computerized image processing system was used to count optic nerve fibers. Results The results of FVEP at 2 weeks after injury showed that the amplitude ratio of FVEP in injured eyes and healthy eyes was 0.765 ± 0.150 in NGF group and 0.494 ± 0.108 in control group, and there was significant difference between NGF group and control group (P < 0.01). At 8 weeks after injury, the amplitude ratio of FVEP in injured eyes to healthy eyes was 0.581 ± 0.138 in NGF group and 0.409 ± 0.119 in control group. There was significant difference between NGF group and control group (P <0.05). Optical microscopy and electron microscopy at 8 weeks after injury showed that the degeneration of RGC and optic nerve fiber in NGF group was lighter than that in control group. At 8 weeks after injury, the number of optic nerve fibers in the NGF group and the control group were (10955 ± 608.7) and (7898 ± 608.8) roots / mm2, respectively. There was significant difference between the two groups (P <0.001). Conclusion NGF can increase the survival of RGC to a certain degree and promote the regeneration of axon. Therefore, NGF can promote the recovery of optic nerve injury and visual function recovery.