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在模拟生理条件下,运用荧光光谱和分子对接方法研究了核苷衍生物6-氯嘌呤(6-CP)与人血清白蛋白(HSA)的相互作用.结果表明,6-CP对HSA内源荧光的猝灭类型为形成复合物的静态猝灭,通过热力学参数确定6-CP与HSA之间存在氢键和范德华力,分子对接结果也证明了氢键和疏水作用力的存在.三维荧光光谱研究了6-CP对HSA构象的影响,并以华法令和布洛芬为分子探针进行竞争实验.在此基础上,以6-CP为分子探针,运用固定波长同步荧光光谱法测定了生物样品中的蛋白质含量.在最佳实验条件下,对11份空白溶液进行平行测定,检测限达到0.511 mg/L,相对标准偏差为0.324%.运用此方法对血清、唾液进行了加标回收实验.
Under simulated physiological conditions, the interaction between 6-chloropurine (6-CP) and human serum albumin (HSA) was studied by fluorescence spectroscopy and molecular docking.The results showed that 6-CP had no effect on HSA endogenous The quenching type of fluorescence is the static quenching of the complex formation. The hydrogen bond and van der Waals forces between 6-CP and HSA are determined by the thermodynamic parameters. The molecular docking results also prove the existence of hydrogen bonding and hydrophobic interaction. The three-dimensional fluorescence spectrum The effect of 6-CP on the conformation of HSA was studied, and warfarin and ibuprofen were used as molecular probes to compete with each other.On the basis of this, 6-CP was used as a molecular probe to determine the bioactivity of HSA by using fixed wavelength synchronous fluorescence spectrometry The protein content in the sample under the best experimental conditions, 11 blank solution parallel determination, the detection limit of 0.511 mg / L, the relative standard deviation of 0.324%. Using this method of serum, saliva spiked recovery experiments .