论文部分内容阅读
目的研究在动脉粥样硬化大鼠不同应激模型中,血液IL-6、CRP水平的变化及意义。方法实验大鼠50只,体重180~220g,按体重随机分为正常组(n=6)和动脉粥样硬化组(n=44),将建立动脉粥样硬化模型的44只动物按体重随机分为3组:无应激组(n=10),生理应激组(n=12),情绪应激组(n=12)。于第10周末次应激后1小时采集血标本,采用放射免疫法和酶联免疫吸附法测定血清IL-6、CRP浓度。结果情绪应激组大鼠血清IL-6(51.80±4.60 pg/ml vs 27.60±4.19pg/ml,8.01±1.39pg/ml,7.83±1.37pg/ml)、CRP(1.39±0.45 vs 0.90±0.29,1.01±0.22,0.71±0.13)浓度较其他各组明显升高(均P<0.05)。结论慢性情绪应激引起血液IL-6、CRP水平升高,且两者之间存在正相关关系,检测血液中炎症指标水平可反映动脉粥样硬化斑块内炎症程度。
Objective To study the changes and significance of blood IL-6 and CRP in different stress models of atherosclerosis rats. Methods Fifty rats weighing 180-220 g were randomly divided into normal group (n = 6) and atherosclerosis group (n = 44). Forty-four animals with atherosclerosis were randomly divided into three groups according to body weight Divided into three groups: no stress group (n = 10), physiological stress group (n = 12), emotional stress group (n = 12). Serum samples were collected 1 hour after the last 10 weeks of stress, and the serum concentrations of IL-6 and CRP were measured by radioimmunoassay and enzyme-linked immunosorbent assay. Results The levels of IL-6 (51.80 ± 4.60 pg / ml vs 27.60 ± 4.19 pg / ml, 8.01 ± 1.39 pg / ml, 7.83 ± 1.37 pg / ml) and CRP (1.39 ± 0.45 vs 0.90 ± 0.29 , 1.01 ± 0.22,0.71 ± 0.13) were significantly higher than those in other groups (all P <0.05). Conclusion Chronic emotional stress causes elevated levels of blood IL-6 and CRP, and there is a positive correlation between the two. The detection of blood levels of inflammation can reflect the degree of inflammation in atherosclerotic plaque.