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目的采用静电纺丝制备淫羊藿苷纳米纤维膜,并通过自组装技术形成纳米囊泡,改善淫羊藿苷在体内的相容性及吸收性能。方法通过溶解度实验筛选合适溶剂,以静电纺丝技术制备淫羊藿苷纳米纤维膜,采用扫描电镜对纤维膜表面形态进行观察,采用X射线晶体衍射(XRD)和差示扫描量热分析(DSC)检测纤维膜中药物的存在状态,通过红外光谱分析药物与纤维材料之间的相互作用。并通过透射电镜观察纳米纤维膜自组装纳米囊泡的性能。结果甲醇与二甲基乙酰胺混合溶剂的溶解性及纤维成型性较好;载药纤维直径分布均匀(400～600 nm)、表面光滑无药物颗粒,药物与聚合物之间通过氢键作用,具有良好的相容性,水中溶解试验发现纳米纤维膜能自组装成纳米囊泡。结论药物以无定形态高度分散于纳米纤维中,电纺制备工艺简单易行;且淫羊藿苷纳米纤维膜能自组装成纳米囊泡。
OBJECTIVE To prepare icariin nanofiber membrane by electrospinning and to form nano-vesicles by self-assembly technique to improve the compatibility and absorption properties of icariin in vivo. Methods Icariin nanofibers were prepared by electrospinning technique through screening the appropriate solvents by solubility test. The surface morphology of the fibers was observed by scanning electron microscopy. The morphology of the fibers was characterized by X-ray diffraction (XRD) and differential scanning calorimetry (DSC) ) To detect the presence of the drug in the fiber membrane and analyze the interaction between the drug and the fiber material by infrared spectroscopy. The nanofibrous membrane self-assembled nanofibers were observed by transmission electron microscopy. The results showed that the solubility of the mixed solvent of methanol and dimethylacetamide was good and the fiber formability was good. The diameter of drug-loaded fibers was uniform (400-600 nm), the surface was smooth and free of drug particles. The drug and polymer were hydrogen- Has good compatibility, water dissolution test found that nanofiber membranes can self-assembled into nano-vesicles. Conclusion The drug is highly dispersed in nanofibers in the amorphous state. The electrospinning process is simple and easy. The icariin nanofibrous membrane can self-assemble into nanofibers.