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以上海主要栽培番茄品种“鲜丰”的下胚轴作为外植体诱导愈伤组织 ,用 Na Cl进行直接高盐胁迫和逐渐加大盐浓度胁迫筛选。研究结果表明 ,逐渐加大 Na Cl浓度胁迫筛选获得的耐盐性大多属生理适应性 ,直接高盐胁迫筛选才有可能获得真正的耐盐突变体。直接高盐胁迫筛选再生出的 1 2株耐盐植株 ,在 1 5 0 mmol/L Na Cl的盐胁迫下 ,幼苗的成活率可达6 6 % ,而未经胁迫筛选过的原始株成活率则为零。其中 ,2株耐盐突变株能正常开花、结果。其后代在盐胁迫培养基中芽体的生根率、鲜重及干重均显著高于原始株
The hypocotyls of “Xianfeng”, a major tomato cultivar in Shanghai, were used as explants to induce callus. NaCI was used to direct salt stress and salt stress was gradually increased. The results showed that most of the salt tolerance obtained by Na Cl concentration stress screening is physiologically adaptive, and it is possible to obtain a true salt tolerance mutant by direct high salt stress screening. Twelve salt-tolerant plants were screened out by direct salt stress. Under the NaCl stress of 150 mmol / L NaCl, the survival rate of seedlings was up to 66%, while the surviving rate of untreated original strain It is zero. Among them, two salt tolerant mutants can flowering normally, the result. The rooting rate, fresh weight and dry weight of buds of their offspring in salt stress medium were significantly higher than those of the original strain