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以在线扫集-胶束毛细管电动色谱法(Sweeping-MEKC)测定了复方氨酚烷胺胶囊中的马来酸氯苯那敏、咖啡因和对乙酰氨基酚3种有效成分。考察了缓冲溶液pH值、SDS浓度、分离电压及进样时间等对分离效果的影响。优化条件:以未涂层熔融石英毛细管(55 cm×50μm,有效柱长35 cm)为分离柱;环境温度25℃;80 mmol/L十二烷基磺酸钠+20 mmol/L NaH2PO4(pH 2.2)+15%乙腈为缓冲体系,分离电压-20kV,进样时间60 s(H=20.0 cm),测量波长210 nm。在该条件下氯苯那敏、咖啡因和对乙酰氨基酚在25min内出峰,峰面积RSD均小于4%;线性范围分别为2.45～39.17、1.61～25.76、1.58～25.28 mg/L;检出限(S/N=3)分别达139、34、24μg/L,回收率分别为96%～101%、98%～102%、96%～102%。
The active ingredients of chlorphenamine maleate, caffeine and paracetamol in compound paracetamol capsules were determined by Sweeping-MEKC. The effects of buffer pH value, SDS concentration, separation voltage and injection time on the separation efficiency were investigated. The optimum conditions were as follows: uncoated fused silica capillary (55 cm × 50 μm, effective column length 35 cm) was used as the separation column at ambient temperature of 25 ℃; 80 mmol / L sodium dodecyl sulfate + 20 mmol / L NaH 2 PO 4 2.2) + 15% acetonitrile buffer system, the separation voltage -20kV, injection time 60s (H = 20.0cm), measurement wavelength 210nm. Under these conditions, the chlorpheniramine, caffeine and paracetamol peaked within 25 min with the RSDs of peak area less than 4%. The linear range was 2.45 ~ 39.17, 1.61 ~ 25.76 and 1.58 ~ 25.28 mg / L respectively. The limits of recovery (S / N = 3) were 139, 34 and 24 μg / L, respectively. The recoveries were 96% -101%, 98% -102% and 96% -102%, respectively.