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烟草(Nicotiana tobacum)中广泛存在萜类物质,他们散发特殊气味,在代谢产物分泌合成、信号传递和防御反应等方面发挥作用,具有重要的生理、生态作用和经济价值。我们通过生物信息学分析对普通烟草TN90基因组序列中的萜类合酶基因TPS进行广泛预测,并分析了其理化性质、基因结构、进化关系、亚细胞定位、表达谱等信息。本研究在栽培烟草TN90中发现了68个TPS基因的候选序列,通过系统发生分析,可将栽培烟草TPS基因家族成员分为TPS-a、TPS-b、TPS-c、TPS-e/f和TPS-g5个亚家族。所有基因至少在叶、茎、花、根中的一种器官中表达。这些研究为烟草TPS基因功能的深入分析提供了理论依据。
Tobacco (Nicotiana tobacum) widespread in the terpenoids, they emit a special odor, secretion of synthetic metabolites, signaling and defense reactions play a role, has important physiological, ecological role and economic value. Through bioinformatics analysis, we widely predicted the terpenoid synthase gene TPS in the genomic sequence of common tobacco TN90 and analyzed its physical and chemical properties, gene structure, evolutionary relationships, subcellular localization and expression profile. TPS-a, TPS-c, TPS-c, TPS-e / f and TPS-a were all classified into six candidate TPS genes in cultivated tobacco TN90 by phylogenetic analysis. TPS-g5 subfamilies. All genes are expressed in at least one of the organs of leaves, stems, flowers and roots. These studies provide a theoretical basis for the further analysis of tobacco TPS gene function.