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输液或大容量注射液中的热原主要来自内毒素,而革兰氏阴性菌外细胞壁碎片的脂多糖,则可用各种吸附剂组成的活性深层滤器滤除。深层滤器的吸附剂有石棉、高岭土、氧化铝及CMF-perlite(将珍珠岩用多价阳离子处理使其Zeta 电位由负转正)等。一些报道认为,深层滤器的过滤效果的差别取决于被过滤的溶液,溶液中电解质的存在可导致过滤效率降低。本文用[~(51)Cr]-内毒素以研究溶液中电解质对吸附剂的影响。所用内毒素为大肠杆菌(E.coli B 111:04)和马流产沙门氏菌(S.ab.acqui),按Braude 法标记,置4℃贮存,并经透析后或与鲎试剂培育后测定[~(51)Cr]-内毒素的稳定性和活性回收率以及用光散射技术测定理化参数。实验证明,[~(51)Cr]-内毒素的活性是非标记内
The pyrogen in infusion or large volume injection is mainly from endotoxin, while the lipopolysaccharide from cell wall fragments outside of Gram-negative bacteria can be filtered off with an active deep filter composed of various adsorbents. Deep filter adsorbents are asbestos, kaolin, alumina and CMF-perlite (the perlite with multivalent cation treatment Zeta potential from negative to positive) and so on. Some reports suggest that the difference in the filtration efficiency of the depth filter depends on the solution being filtered, and the presence of electrolyte in the solution can lead to a decrease in filtration efficiency. In this paper, [~ (51) Cr] - endotoxin was used to study the effect of electrolytes on the adsorbents in solution. Endotoxins used were Escherichia coli (E. coli B 111: 04) and S. abacaci, which were labeled according to the Braude method and stored at 4 ° C. After dialysis or incubation with the testis, (51) Cr] - endotoxin stability and activity recovery as well as the use of light scattering techniques to determine physical and chemical parameters. Experiments show that [~ (51) Cr] - endotoxin activity is non-labeled