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目的 :探讨儿童自身免疫甲状腺病 (AITD)细胞因子失衡与T细胞亚群失衡的关系。方法 :采用E花环法和ELISA法同步分别检测初诊的AITD患儿和正常儿童外周血T细胞亚群和单个核细胞在丝裂原诱导下产生的IFN -γ、IL - 4和IFN -γ IL - 4比值。结果 :与正常组相比 ,CLT和GD病儿IFN -γ水平和IFN -γ IL - 4比值明显升高 ,IL - 4水平明显降低 ,CD+3、CD+8明显降低 ,CD+4 CD+8比值明显升高。且CD+8与IL - 4水平、CD+4 CD+8比值与IFN -γ IL - 4比值呈显著正相关 (r分别为 0 .76 0 .75 ,0 .6 8 0 .5 8,P <0 .0 5 )。结论 :AITD患儿Th1类细胞因子升高可能主要是CD+4Th1亚类的功能亢进所致 ,而Th2类细胞因子的降低可能主要是CD+8Th2亚类数量和功能降低所致
Objective: To investigate the relationship between imbalance of cytokines and imbalance of T lymphocyte subsets in children with autoimmune thyroid disease (AITD). Methods: T - lymphocyte subsets and mononuclear cells from newly diagnosed AITD children and normal children were detected by mitochondria - induced syncytiotrophoblast IFN - γ, IL - 4 and IFN - γ IL - 4 ratio. Results: Compared with the normal group, the levels of IFN - γ and the ratio of IFN - γ and IL - 4 in CLT and GD sick children were obviously increased, while the levels of IL - 4 and CD + 3 and CD 8 were significantly decreased +8 ratio increased significantly. There was a significant positive correlation between the ratio of CD + 8 and IL - 4, the ratio of CD + 4 CD + 8 and the ratio of IFN - γ IL - 4 (r = 0.76 0 .75,0.680.58, P <0 .0 5). Conclusion: Th1 type cytokines in children with AITD may be mainly due to hyperactivity of CD + 4Th1 subclass, and the decrease of Th2 type cytokines may be mainly caused by the decrease in the number and function of CD + 8Th2 subclasses