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目的:研究金雀异黄素和槲皮素对HSC-T6大鼠肝星状细胞增殖和胶原合成及Ⅰ型原胶原mRNA表达的影响。方法:细胞增殖和胶原合成分别采用结晶紫染色法和[~3H]-脯氨酸掺入法。原胶原mRNA水平用RT-PCR法测定。结果:金雀异黄素(25-70μmol/L)和槲皮素(6.25-50μmol/L)浓度依赖抑制HSC-T6细胞增殖和胶原合成,对Ⅰ型原胶原mRNA表达也有抑制作用。结论:金雀异黄素和槲皮素抑制肝星状细胞增殖和胶原合成可能对肝纤维化有保护作用。
Objective: To investigate the effects of genistein and quercetin on proliferation, collagen synthesis and type I procollagen mRNA expression in HSC-T6 rat hepatic stellate cells. METHODS: Crystal violet staining and [~3H]-proline incorporation were used for cell proliferation and collagen synthesis, respectively. The procollagen mRNA levels were determined by RT-PCR. RESULTS: Genistein (25-70 μmol/L) and quercetin (6.25-50 μmol/L) concentration-dependently inhibited HSC-T6 cell proliferation and collagen synthesis, and also inhibited the expression of type I procollagen mRNA. CONCLUSION: The inhibitory effects of genistein and quercetin on hepatic stellate cell proliferation and collagen synthesis may have protective effects on hepatic fibrosis.