Objective: To evaluate the management of early-onset sepsis at Saad Abul-Ella Teaching Hospital, Sudan. Methods: A descriptive retrospective hospital-based study was carried out at the Nursery Department of Saad Abul-Ella Teaching Hospital. All medical records of neonates with suspected or confirmed sepsis during the year 2017 were reviewed to evaluate the management of antibiotics for sepsis using a data collection form. Results: Out of the 205 cases, 82 neonates (40％) were diagnosed as early-onset sepsis, among which the majority was male (68％). All neonates were given cefotaxime plus vancomycin as empirical therapy which was changed to other antibiotics in 23％ of the cases. The common risk factors associated with early-onset sepsis wereprolonged rupture of membrane (41.8％), preterm delivery (26.3％) and low birth weight (15.1％). Blood cultures were performed in 168 cases, and 19％ had bacterial growth of Staphylococcus aureus which is the most common isolated pathogen. Conclusions: Cefotaxime plus vancomycin are the main empirical antibiotic for sepsis, and Staphylococcus aureus is the most common pathogen associated with early-onset sepsis.