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为探讨茶色素对高胆固醇兔动脉粥样硬化的影响 ,将 2 4只新西兰雄性白兔按血脂水平随机分为对照组、茶色素低剂量组 [32 μg (g·d) ]和高剂量组 [96 μg (g·d) ],每组 8只 ,实验期间均以高胆固醇饮食。喂养 8周后处死动物 ,测定血脂水平、血浆内皮素水平和斑块面积比。结果发现 ,实验 4周及 8周后血清总胆固醇和甘油三酯水平明显高于实验前 ,但三组间没有明显差异。实验 8周后对照组、茶色素低剂量组和高剂量组血浆内皮素水平分别为 775 .5 8± 6 6 8.72 μg L、2 5 1.73± 2 41.5 7μg L和 6 5 .43± 17.18μg L。与对照组相比 ,茶色素低剂量组内皮素水平有所降低 ,但无显著性差异 (P >0 .0 5 ) ,茶色素高剂量组内皮素水平明显降低 (P <0 .0 5 )。对照组、茶色素低剂量组和高剂量组动物胸主动脉斑块面积百分比分别为 0 .793± 0 .0 77、0 .5 88± 0 .2 0 5和 0 .437± 0 .116 ,内膜面积与中膜面积百分比分别为 0 .6 2 5± 0 .2 0 6、0 .32 6± 0 .2 76和 0 .175± 0 .0 92 ,茶色素组斑块面积明显低于对照组 (P <0 .0 5 )。结果提示 ,茶色素能够防止兔实验性粥样斑块的形成 ,并降低血浆内皮素水平
To investigate the effect of tea pigment on atherosclerosis in hypercholesterolemic rabbits, 24 male New Zealand white rabbits were randomly divided into control group, low-dose tea pigment group [32 μg (g · d)] and high-dose group [96 μg (g · d)], with 8 rats in each group. All of them had high cholesterol diet during the experiment. Animals were sacrificed 8 weeks after feeding, blood lipid levels, plasma endothelin levels and plaque area ratio were determined. The results showed that serum total cholesterol and triglyceride levels were significantly higher 4 weeks and 8 weeks after the experiment, but there was no significant difference between the three groups. After 8 weeks of experiment, the levels of plasma endothelin in the control group, low-dose tea group and high-dose tea group were 775.58 ± 66.72 μg L, 25.173 ± 21.57μg L and 65.34 ± 17.18μg L . Compared with the control group, the level of endothelin in the low-dose tea pigment group decreased but there was no significant difference (P> 0.05), and the endothelin level in the high-dose tea pigment group decreased significantly (P <0.05) . The area of thoracic aortic plaques in the control group, low-dose tea group and high-dose group were 0.793 ± 0.777.588 ± 0.250 and 0.437 ± 0.116, Intimal area and medial area percentages were respectively 0.62 ± 0.26,0.332 6 ± 0.276 and 0.751 ± 0.92, and the area of plaque in tea pigment group was significantly lower than Control group (P <0.05). The results suggest that tea pigment can prevent the formation of experimental atherosclerotic plaque and reduce the plasma endothelin level