【摘 要】
：
The effect of varying pH on the photosystem II (PSII) membrane was studied using absorption and steady-state fluorescence spectroscopy, and using a variable flu
【机 构】
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State Key Laboratory of Biomembrane and Membrane Biotechnology,Photosynthesis Research Center
【基金项目】
：
国家自然科学基金;国家重点基础研究发展计划(973计划)
论文部分内容阅读
The effect of varying pH on the photosystem II (PSII) membrane was studied using absorption and steady-state fluorescence spectroscopy, and using a variable fluorescence technique.pH variations induced significant changes in the chlorophyll states of the PSII membrane, but no effect was seen on the chlorophyll fluorescence parameter F′v/F′m.For acidic pH conditions, protein structures of the PSII membrane were slightly altered, whilst at alkaline pH levels, large changes in the protein structure of the PSII membrane were detected.The results indicate that the microenvironment around Cys in the PSII membrane is very susceptible to alkaline pH conditions, and that in the acid (4≤pH7) regions, pH variation has no effect on the protein structures of the PSII reaction center (RC).
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