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目的比较转移与无转移肝细胞癌(HCC)患者的血清蛋白质指纹图谱,筛选与肝癌转移相关的蛋白质。方法术前收集84例HCC患者(有转移45例,无转移39例)和30例健康成人的血清,采用弱阳离子交换蛋白质芯片为检测介质,经表面加强激光解吸电离-飞行时间-质谱(SELDI-TOF-MS)测定,得到蛋白质指纹图谱并运BioMarkerWizard软件分析比较。结果在质荷比(m/z)1500~30000范围内,比较有转移HCC与健康成人的血清,检测出的146个蛋白质峰中,69个蛋白质峰差异有统计学意义(P<0.05),比较无转移HCC与健康成人的血清,60个蛋白质峰差异有统计学意义(P<0.05),差异蛋白质峰中57个为有转移与无转移HCC的共有蛋白峰;单独比较有转移与无转移HCC血清,检测出的146个蛋白质峰中仅有11个蛋白质峰差异有统计学意义(P<0.05)。结论有转移与无转移HCC患者血清蛋白质之间有差异,但远较其与健康成人血清之间差异小;血清蛋白质指纹图谱中的差异蛋白质可能与肿瘤的发生和转移有关。
Objective To compare serum protein fingerprints in patients with metastatic and non-metastatic hepatocellular carcinoma (HCC) and screen for proteins associated with liver cancer metastasis. Methods Serum samples of 84 HCC patients (45 cases with metastasis, 39 cases without metastasis) and 30 healthy adults were collected preoperatively and were detected by SELDI-TOF-MS using weak cation-exchange protein chip as detection medium. -TOF-MS), the protein fingerprinting was obtained and analyzed by BioMarkerWizard software. Results The serum levels of HCC in metastatic HCC and healthy adults were compared in the range of 1,500 ~ 30,000 with mass / charge ratio (m / z). Among the 146 detected protein peaks, the peak value of 69 proteins was statistically significant (P <0.05) There were significant differences in 60 protein peaks between the serum of patients without metastasis HCC and healthy adults (P <0.05), and 57 of the differential protein peaks were shared protein peaks with and without metastasis In HCC serum, only 11 of the 146 protein peaks detected had significant difference (P <0.05). Conclusions There is a difference between the serum proteins of metastatic and non-metastatic HCC patients, but far less than that of serum of healthy adults. The differential proteins in serum protein fingerprinting may be related to tumorigenesis and metastasis.