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目的:通过观察藏红花对肝纤维化大鼠肝组织TGF-β1表达的影响,探讨藏红花预防肝纤维化作用的机制。方法:清洁级SD雄性大鼠60只随机分为正常组、模型组、藏红花组、丹参组,每组十五只。应用30%四氯化碳橄榄油溶液3ml/kg.腹腔注射制备肝纤维化大鼠模型。治疗8周后,通过HE和Masson染色观察肝纤维化的形成、免疫组化检测肝组织中TGF-β1蛋白的表达。结果:臧红花组、丹参组与模型组相比肝纤维化程度均轻于模型组(P<0.01),而藏红花组与丹参组相比肝纤维化程度无显著性差异(P>0.05),臧红花组、丹参组与模型组相比TGF-β1蛋白的表达均明显减少,而藏红花组与丹参组相比TGF-β1蛋白的表达没有显著性差异(P>0.05)。结论:藏红花能有效减轻肝纤维化大鼠的肝脏损伤及纤维化程度,其机制可能与抑制肝内TGF-β1的表达,抑制HSC的激活以及阻断HSC与TGF-β1之间的恶性循环有关。
Objective: To observe the effect of saffron on the expression of TGF-β1 in hepatic tissue of rats with hepatic fibrosis and explore the mechanism of saffron in preventing hepatic fibrosis. Methods: Sixty clean SD male rats were randomly divided into normal group, model group, saffron group and Salvia miltiorrhiza group, with 15 in each group. Application of 30% carbon tetrachloride olive oil solution 3ml / kg. Preparation of hepatic fibrosis rat model by intraperitoneal injection. After 8 weeks of treatment, the formation of liver fibrosis was observed by HE and Masson staining, and the expression of TGF-β1 protein in liver tissue was detected by immunohistochemistry. Results: Compared with the model group, Zanghonghua group and Salvia miltiorrhiza group showed less liver fibrosis than the model group (P <0.01), while there was no significant difference in the degree of liver fibrosis between the saffron group and the Salvia miltiorrhiza group (P> 0.05) Compared with the model group, the expression of TGF-β1 protein in Salvia miltiorrhiza group and Salvia miltiorrhiza group were significantly decreased, while the expression of TGF-β1 protein in Saffron group and Salvia miltiorrhiza group was not significantly different (P> 0.05). Conclusion: Saffron can effectively reduce liver injury and fibrosis in rats with hepatic fibrosis. The mechanism may be related to inhibiting the expression of TGF-β1 in the liver, inhibiting the activation of HSC and blocking the malignant cycle between HSC and TGF-β1 .