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目的 研究人胃癌基因组中Cx基因的表达、探讨诱导剂作用后Cx基因的表达变化及Cx43编码序列有无突变。方法 Northern杂交、RT PCR和PCR 单链构象多肽分析。结果 1.发现了在人正常胃黏膜上皮、癌旁组织及胃癌中Cx基因的表达规律 ,明确了Cx基因在人胃癌基因组中的表达谱。 2 .维甲酸 (RA)、二甲基亚砜 (DMSO)对胃癌细胞基因组中Cx43基因有诱导表达作用 ,而其本身表达的Cx46在RA及佛波酯 (TPA)作用后明显减弱或消失。 3 .Cx43基因编码区未发现突变。结论 1.Cx32可能是人胃上皮细胞基因组中维持细胞间隙连接通讯功能的特异表达的Cx基因 ,Cx46可能是胃癌Cx基因表达的一种变异。 2 .Cx43基因在胃癌细胞中有可诱导性。 3 .Cx43基因在人胃癌中表达下调的原因不是其编码区的点突变。 4.本文提出了有关人胃癌中Cx基因表达变异的假说
Objective To study the expression of Cx gene in the human gastric cancer genome, to investigate the expression of Cx gene and the presence or absence of mutations in the Cx43 coding sequence. Methods Northern hybridization, RT PCR and PCR single-stranded conformational polypeptide analysis. Results 1. The expression pattern of Cx gene in human normal gastric epithelium, paracancerous tissues, and gastric cancer was found, and the expression profile of Cx gene in human gastric cancer genome was clarified. 2. Retinoic acid (RA) and dimethyl sulfoxide (DMSO) induced the expression of Cx43 gene in the genome of gastric cancer cells, but the Cx46 itself expressed in the gastric cancer cells weakened or disappeared after the action of RA and phorbol ester (TPA). 3. No mutation was found in the coding region of the Cx43 gene. Conclusion 1. Cx32 may be the specific expression of Cx gene in the genome of human gastric epithelial cells that maintains cell gap junctional communication function. Cx46 may be a variant of gastric cancer Cx gene expression. 2. The Cx43 gene is inducible in gastric cancer cells. 3. The reason for the down-regulation of Cx43 gene in human gastric cancer is not the point mutation in its coding region. 4. This article presents a hypothesis about the variation of Cx gene expression in human gastric cancer.