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目的探讨铝螯合剂1,2-二甲基-3-羟基-4-吡啶酮(1,2-dimethyl-3-hydroxy-4-pyridone,DFP)对染铝大鼠睾丸组织中抗氧化系统及铝、铁、锌、钙、镁、铜元素的影响。方法将35只健康清洁级雄性Wistar大鼠随机分为5组,分别为阴性对照组(生理盐水)、低、中、高剂量DFP+Al组和阳性对照组。前8周,低、中、高剂量DFP+Al组和阳性对照组均给予172.5mg/(kg·d)的氯化铝溶液。后3周,阳性对照组给予1ml生理盐水,低、中、高剂量DFP+Al组分别给予剂量为13.82、27.44和54.88mg/(kg·d)的DFP溶液。给药均采用灌胃方式染毒,每周连续5d,间隔2d后继续给药。染毒后24h,取出睾丸,称重,并计算睾丸系数。测定各组睾丸组织中超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量以及铝、铁、锌、钙、镁、铜元素的含量。结果各组大鼠体重、睾丸重量和睾丸系数间比较,差异均无统计学意义(P>0.05)。随着DFP染毒剂量的升高,睾丸组织中SOD活力呈上升趋势,MDA含量呈下降趋势。与阳性对照组相比,高剂量DFP+Al组睾丸组织中SOD活力较高,MDA含量较低,差异均有统计学意义(P<0.05或P<0.01)。高、中、低剂量DFP+Al组睾丸组织中SOD活力和MDA含量与阴性对照组相比,差异无统计学意义(P>0.05)。高、中、低剂量DFP+Al组和阴性对照组大鼠睾丸组织中的铝含量均低于阳性对照组,差异有统计学意义(P<0.01)。各组大鼠睾丸组织中Fe、Zn、Ca、Mg、Cu元素含量间比较,差异均无统计学意义(P>0.05)。结论DFP对染铝大鼠受损睾丸组织抗氧化系统有保护作用,且在排铝的同时,对睾丸组织中必需元素铁、锌、钙、镁、铜的代谢平衡无影响。
Objective To investigate the effects of 1,2-dimethyl-3-hydroxy-4-pyridone (DFP), an aluminum chelator, Effect of aluminum, iron, zinc, calcium, magnesium and copper. Methods 35 healthy male Wistar rats were randomly divided into 5 groups: negative control group (saline), low, middle and high dose DFP + Al group and positive control group. The first 8 weeks, low, medium and high doses of DFP + Al group and positive control group were given 172.5mg / (kg · d) of aluminum chloride solution. After 3 weeks, the positive control group was given 1 ml of normal saline, and the low, medium and high doses of DFP + Al were given DFP solution at dosage of 13.82, 27.44 and 54.88 mg / (kg · d) respectively. Administered by gavage administration, weekly for 5d, continue after 2d administration. After exposure for 24 hours, testis was removed, weighed, and testicular coefficient calculated. The activities of superoxide dismutase (SOD) and malondialdehyde (MDA) and contents of aluminum, iron, zinc, calcium, magnesium and copper in testis tissue of each group were determined. Results There was no significant difference in body weight, testicular weight and testicular coefficient among all groups (P> 0.05). With the increase of dose of DFP, the activity of SOD in testis increased and the content of MDA decreased. Compared with the positive control group, the activity of SOD and the content of MDA in testis of high dose DFP + Al group were significantly lower (P <0.05 or P <0.01). Compared with the negative control group, there was no significant difference in SOD activity and MDA content between testis tissues of high, medium and low dose DFP + Al groups (P> 0.05). The contents of aluminum in the testes of DFP + Al group and the negative control group were lower than those of the positive control group (P <0.01). The contents of Fe, Zn, Ca, Mg and Cu in testis of rats in each group were not significantly different (P> 0.05). Conclusion DFP has a protective effect on the antioxidant system of damaged testis tissue in aluminum-exposed rats, and has no effect on the metabolic balance of iron, zinc, calcium, magnesium and copper in testis tissue while aluminum is discharged.