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目的通过免疫组织化学方法,观察大鼠卵巢切除术(OVX)后正畸牙周组织内TNFβ的表达,了解雌激素缺乏时正畸牙周组织的改建情况。方法将OVX及对照组SD大鼠按术后的天数随机分为0、7、21、42d组,分别施以相同的矫治装置,加力2周,力值40g,牵引大鼠第1磨牙向近中移动。实验结束后处死动物,取上颌正畸牙及其牙周组织作免疫组化染色。结果OVX组大鼠正畸牙周组织内TNFβ呈强阳性表达,而对照组大鼠的相同部位TNFβ则呈阴性表达,主要阳性表达细胞为牙周成纤维细胞、破骨细胞;随着OVX术后时间的延长,实验组大鼠牙周组织内TNFβ的含量逐渐增加,两组切片染色的灰度值差异有非常显著意义(P<001)。结论雌激素缺乏时正畸牙周组织改建有其特殊性,TNFβ在OVX大鼠正畸牙周组织的改建中有重要作用,其机制尚需进一步研究。
Objective To observe the expression of TNFβ in periodontal tissues of rats after ovariectomy (OVX) by immunohistochemistry and to understand the reconstruction of periodontal tissues during the period of estrogen deficiency. Methods The rats of OVX group and control group were randomly divided into 0, 7, 21 and 42 d groups according to the number of days after operation. The same orthodontic appliance was used for 2 weeks and the force value was 40 g. Move near. Animals were sacrificed after the experiment, and the maxillary orthodontic teeth and periodontal tissues were taken for immunohistochemical staining. Results The expression of TNFβ in periodontal tissues of OVX rats was strongly positive, while the expression of TNFβ in the same parts of rats in control rats was negative. The main positive cells were periodontal fibroblasts and osteoclasts. With OVX After prolonged, the content of TNFβin the periodontal tissue of the experimental group increased gradually, and the difference of the gray value of the two groups was very significant (P <001). Conclusion Orthodontic periodontal tissue remodeling has its particularity in the absence of estrogen. TNFβ plays an important role in orthodontic periodontal remodeling in OVX rats, and its mechanism needs further study.