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反相薄层色谱(RPTLC)中使用两类固定相:(1)物理结合的,(2)化学键合的。前者的制备常用石蜡油等非极性液体涂在硅胶等载体上,此固定相的缺点是稳定性和重现性较差。化学键合固定相具有许多优点;斑点扩散小;R值重现性好;点样量较大;能广泛用于多种样品的分离,特别适于极性强的复杂样品(氨基酸、维生素等)。化学键合固定相的出现使RPTLC近几年得到迅速发展。本文综述化学键合相TLC的最新进展。
Two types of stationary phases are used in reverse phase thin layer chromatography (RPTLC): (1) physically bound, (2) chemically bound. Preparation of the former commonly used non-polar liquid paraffin oil and other carriers coated on silica gel, the disadvantage of this stationary phase is poor stability and reproducibility. Chemically bonded stationary phases have many advantages; small spot diffusion; good reproducibility of R values; large sample volume; wide range of applications for the separation of a wide range of samples, especially for highly complex and complex samples (amino acids, vitamins, etc.) . The appearance of chemically bonded stationary phases has led to the rapid development of RPTLC in recent years. This article reviews the latest developments in chemically coupled phase TLC.