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目的研究碳纤维复合材料粉尘(CFC)的细胞毒性作用。方法以小鼠肺成纤维细胞(NIH/3T3)为靶细胞,以某飞机制造企业使用的碳纤维复合材料沉降尘为受试物,采用噻唑蓝(MTT)比色法检测碳纤维复合材料粉尘对NIH/3T3细胞活性与增殖的影响,并计算相对增殖率(RGR);采用单细胞凝胶电泳(SCGE)技术检测NIH/3T3细胞的DNA损伤情况。结果经受试物染毒的细胞数目减少,有死亡细胞,随着染毒浓度的增加,存活的细胞数逐渐减少,并出现细胞吸附毒物的现象。不同浓度下的碳纤维复合材料粉尘(100、200、400、800μg/ml)作用一定时间后,NIH/3T3细胞存活率下降,呈现出时间-剂量-反应关系。碳纤维复合材料粉尘可引起NIH/3T3细胞DNA断裂,染毒组的细胞拖尾率、尾部DNA含量、彗星细胞的Olive尾距、延伸的尾动差均增高,与阴性对照组比较,差异有统计学意义(P<0.05)。结论碳纤维复合材料粉尘具有明显的细胞毒性,并对DNA有损伤作用。
Objective To study the cytotoxicity of carbon fiber composite dust (CFC). Methods Mouse lung fibroblasts (NIH / 3T3) were used as target cells. Carbon fiber composite dust precipitated by some aircraft manufacturers was used as test substance. MTT colorimetry was used to detect the effect of carbon fiber composite dust on NIH / 3T3 cells, and calculate the relative proliferation rate (RGR). The DNA damage of NIH / 3T3 cells was detected by single cell gel electrophoresis (SCGE). Results The number of cells exposed to the test substance decreased, and the number of dead cells decreased. As the concentration increased, the number of surviving cells decreased gradually and cells adsorbed the toxic substances. After a certain period of time, the survival rate of NIH / 3T3 cells decreased and showed a time-dose-response relationship under different concentrations of carbon fiber composite dust (100,200,400,800μg / ml). Carbon fiber composite dust can cause DNA breakage in NIH / 3T3 cells, and the tailing rate, tail DNA content, Olive tail distance of comet cells and tail tails of extension are all higher than those in the negative control group Significance (P <0.05). Conclusion Carbon fiber composite dust has obvious cytotoxicity and DNA damage.