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在联合化疗的急性白血病儿童中肝脏损害常见,除药物对肝脏的直接毒性作用外,病毒性肝炎被认为是引起这种肝脏损害的重要原因。本文作者在意大利米兰大学儿科103例急性白血病患儿中,选择了伴慢性肝病(转氨酶增高3倍,持续6个月以上)肝脏中HBsAg阴性的11例急性淋巴细胞白血病儿童,在停止治疗期间对非甲非乙型(NANB)肝炎感染有关的抗原抗体系统进行了研究。11例患儿中,8例男性,3例女性。年龄4～14岁(平均8.6岁)。化疗时间30～87个月(中位数45个月)。9例在抗白血病治疗初期接受过1～7次输血,2例从未输血。研究与方法按Alberti等法在患儿的血清和肝脏中研究与NANB肝炎有关的抗原抗体系统。通过间接免疫荧光技术用NANB抗原阳性的肝脏作为基质检测血清中NANB抗体。通过直接的免疫荧光技术在11例儿童的肝活检标本中寻找NANB抗原,低温切片(厚4μm)未加固定,用荧光素结合的NANB抗体在37℃染色45分钟(从患输血后NANB,肝炎距其急性发病后8个月康复的病人身上获得的抗血清用来制备
Liver damage is common in children with acute leukemia combined with chemotherapy, and in addition to the direct toxic effects of the drug on the liver, viral hepatitis is considered to be an important cause of this liver damage. The authors selected 11 acute lymphoblastic leukemia children with HBsAg-negative liver in the liver of 103 children with acute leukemia at the University of Milan, Italy, who had chronic liver disease (3-fold more aminotransferase, lasting more than 6 months). During treatment discontinuation Antigen-antibody systems related to non-A, B (NANB) hepatitis infection were studied. Of the 11 children, 8 were males and 3 were females. Age 4 to 14 years (mean 8.6 years). Chemotherapy time was 30 to 87 months (median 45 months). Nine patients received 1 to 7 transfusions in the early stage of anti-leukemia treatment and 2 never transfused. Research and Methods According to the Alberti et al. Method, the antigen-antibody system related to NANB hepatitis was studied in the serum and liver of children. NANB antibodies in serum were detected by indirect immunofluorescence using NANB antigen-positive liver as a substrate. NANB antigen was searched for by direct immunofluorescence in liver biopsy specimens of 11 children. Cold sections (thickness 4 μm) were not fixed and stained with fluorescein-conjugated NANB antibody for 45 minutes at 37 ° C (from NANB after transfusion, hepatitis Antisera obtained from patients recovered from their acute onset of 8 months were used to prepare