论文部分内容阅读
目的 探讨发育期营养不良伴发癫持续状态对海马神经发生的影响。方法 采用5 溴脱氧尿苷 (BrdU)标记新生细胞 ,β微管蛋白Ⅲ ( βⅢtubulin ,TuJ1)和胶质原纤维酸性蛋白(glialfibrillaryacidicprotein ,GFAP)分别标记早期神经元和胶质细胞的单、双标免疫组织化学染色 ,观察幼鼠海马的神经发生。结果 营养良好 (N)组和营养不良 (M)组幼鼠癫发作的潜伏时间差异无显著性 [( 12 4± 2 6)min与 ( 11 9± 2 9)min ,P >0 0 5 ]。组织学染色示各组幼鼠海马部位均无明显的神经元丢失。各组幼鼠齿状回BrdU阳性细胞数 ,营养不良 +惊厥组 (MS组 ,3 74± 18)和营养良好 +惊厥组 (NS组 ,3 12± 2 4)分别明显高于营养不良组 (M组 ,3 0 3± 2 0 )和营养良好组 (N组 ,2 69± 18) (P均 <0 0 1) ,而M组和MS组分别明显高于N组和NS组 (P均 <0 0 1)。BrdU阳性细胞中 ,约有 60 %的BrdU阳性细胞同时表达神经元特异性标记物TuJ1,5 %～ 10 %的BrdU阳性细胞同时表达神经胶质特异性标记物GFAP。结论 早期营养不良没有改变幼鼠在海藻酸致过程中惊厥的易感性和行为表现。营养不良和癫持续状态均可促进幼鼠海马齿状回新生细胞的增殖 ,营养不良伴发癫持续状态时这种增殖将进一步加强 ,而且大多数新生细胞分化为早期?
Objective To investigate the effect of malnutrition with status epilepticus on the hippocampal neurogenesis in developmental stage. Methods BrdU-labeled neoplasms, β-tubulin (TuJ1) and glial fibrillary acidic protein (GFAP) were used to label single and double-stained cells of early neurons and glial cells Immunohistochemical staining was performed to observe the neurogenesis of hippocampus in young rats. Results There was no significant difference in the latency of epileptic seizures in young group with good nutrition (N) and malnutrition (M) [(12 4 ± 2 6) min vs (11 9 ± 29) min, P> 0.05 ]. Histological staining showed no neuronal loss in the hippocampus of each group of young rats. The number of BrdU positive cells, malnutrition + convulsion group (MS group, 3 74 ± 18) and nourishment + convulsion group (NS group, 312 ± 2 4) in each group were significantly higher than those in malnutrition group (P <0.01), while those in M group and MS group were significantly higher than those in N group and NS group (P <0.05) <0 0 1). BrdU-positive cells, about 60% of BrdU-positive cells simultaneously express neuron-specific marker TuJ1, 5% to 10% of BrdU-positive cells while expressing glial-specific marker GFAP. Conclusions Early malnutrition did not change the susceptibility and behavior of convulsions in young rats induced by alginic acid. Malnutrition and status epilepticus can promote the hippocampal dentate gyrus neonatal cell proliferation, malnutrition associated with status epilepticus, this proliferation will be further strengthened, and most of the newborn cells differentiated into early?