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目的:探讨变形链球菌(S.mutans)唾液结合区段(SBR)作为亚单位防龋疫苗的免疫原性,并评价经涎腺接种诱导有效免疫应答的可行性。方法:构建含S.mutansSBR基因的重组原核表达质粒pTriEx-4-SBR,用该质粒转化大肠杆菌E.coliJM109DE3,培养转化后的E.coli并诱导其表达SBR蛋白;以SBR蛋白作为亚单位防龋疫苗经颌下腺免疫BALBC小鼠,ELISA检测特异性抗体指标。结果:纯化得到的SBR蛋白与预期相符;ELISA结果表明,SBR蛋白经颌下腺免疫小鼠能够诱导血清抗SBR特异IgG(P<0.01)、唾液抗SBR特异IgGP<0.01、唾液抗SBR特异IgAP<0.01显著应答。结论:本实验所表达及纯化的SBR蛋白经涎腺接种途径可诱导明显的免疫应答。
Objective: To investigate the immunogenicity of S. mutans salivary junction segment (SBR) as a subunit anti-caries vaccine and to evaluate the feasibility of salivary gland vaccination to induce an effective immune response. Methods: Recombinant prokaryotic expression plasmid pTriEx-4-SBR containing S.mutansSBR gene was constructed. E.coli JM109DE3 was transformed into Escherichia coli E.coli JM109DE3. The transformed E. coli was induced to express SBR protein. SBR protein was used as subunit BALB / c mice were immunized with cariogenic vaccine through submandibular gland, and specific antibodies were detected by ELISA. Results: The purified SBR protein was in accordance with the expectation. The results of ELISA showed that SBR specific anti-SBR specific IgA was less than 0.01 in saliva anti-SBR and 0.01 Significant response. Conclusion: The SBR protein expressed and purified in this experiment can induce a significant immune response via salivary gland inoculation.