Natural product evodiamine and its derivatives represent a promising class of multi-target antitumor agents.However,the clinical development of these compounds has been hampered by a poor understanding of their antitumor mechanisms.To tackle this obstacle,herein,novel fluorescent probes were designed to elucidate the antimmor mode of action of 10-hydroxyevodiamine.This compound was proven to be distributed in the mitochondria and lysosomes and to act by autophagy and apoptosis mechanisms.