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Objective To investigate the effect of α-zearalenol on angiotensin II-induced β3 integrin mRNA expression in human umbilical vein endothelial cells (HUVECs). Methods The mRNA level in integrin β3 was determined by reverse transcription–polymerase chain reaction. Endothelial NF-κB activity was determined by the luciferase activity assay of plasmid NF-κB-LUC. Results The angiotensin II- induced β3 integrin mRNA expression was inhibited by α-zearalenol and 17β-estradiol (10 nmol/L -1 μmol/L), but not influenced by ICI 182, 780, a pure competitive antagonist for estrogen receptor or a nitric oxide inhibitor Nω-Nitro-L-arginine methyl ester hydrochloride. Alpha-zearalenol and 17β-estradiol suppressed the angiotensin II-induced activation of NF-κB in endothelial cells. Conclusion Alpha-zearalenol inhibits angiotensin II-induced integrin β3 mRNA expression by suppressing NF-κB activation in endothelial cells.
Objective To investigate the effect of α-zearalenol on angiotensin II-induced β3 integrin mRNA expression in human umbilical vein endothelial cells (HUVECs). Methods The mRNA level in integrin β3 was determined by reverse transcription-polymerase chain reaction. Endothelial NF-κB activity was determined by the luciferase activity assay of plasmid NF-κB-LUC. Results The angiotensin II-induced β3 integrin mRNA expression was inhibited by α-zearalenol and 17β-estradiol (10 nmol / L -1 μmol / L), but not influenced by ICI 182, 780, a pure competitive antagonist for estrogen receptor or a nitric oxide inhibitor Nω-Nitro-L-arginine methyl ester hydrochloride. Alpha-zearalenol and 17β-estradiol suppressed the angiotensin II-induced activation of NF-κB in endothelial cells . Conclusion Alpha-zearalenol inhibits angiotensin II-induced integrin β3 mRNA expression by suppressing NF-κB activation in endothelial cells.