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上海郊区青菜病毒病为害严重,又较难诊断。经研究,目前已知的青菜病毒病有长叶车前花叶病毒、芜菁花叶病毒和黄瓜花叶病毒三种。本报道以长叶车前花叶病毒为抗原,从免疫兔子得到抗血清及其γ-球蛋白,用过氧化物酶作交联酶制得酶标液,用以快速检测青菜病毒病材料和方法(一)病毒抗原的纯化:将温室人工接种的长叶车前花叶病毒(Ribgrass Mosaic V-irus Holmes 简称 HRV)的青菜病叶放入捣碎机,加等量(pR7.4～7.6)0.4M 的磷酸缓冲液,然后捣碎,用双层纱布挤出汁液,在汁液中滴加8%正丁醇,搅拌半小时后,将
Shanghai suburbs of vegetables serious damage to vegetables, but also more difficult to diagnose. After the study, there are three known diseases of the vegetable virus such as the long-leaved mosaic virus, the turnip mosaic virus and the cucumber mosaic virus. In this report, long-leaf car mosaic virus as an antigen from the immune rabbit to obtain antiserum and γ-globulin, peroxidase enzyme enzyme solution for the preparation of cross-linked enzyme for rapid detection of vegetable disease virus materials and Methods (1) Purification of virus antigen: The diseased cabbage leaves of Ribgrass Mosaic V-irus Holmes artificially inoculated with greenhouse were placed in a stamp mill with the same amount of pR7.4 ~ 7.6 ) 0.4M phosphate buffer, and then mashed, with a double layer of gauze out of juice, the juice was added dropwise 8% n-butanol, stirred for half an hour, will