论文部分内容阅读
分别用不同浓度(50、100、200mg·L-1)的除草剂安全剂类似化合物乙酰苯胺和2-氯苯磺酰胺处理IN2-2::GUS转基因芥菜。结果显示:乙酰苯胺和2-氯苯磺酰胺处理后,IN2-2启动子在芥菜根、叶、花器官的花萼、花瓣、雄蕊及花粉中表达,但不在胚珠中表达。100mg·L-1乙酰苯胺以及50mg·L-12-氯苯磺酰胺适合用于在芥菜中调控IN2-2启动子的表达,乙酰苯胺较2-氯苯磺酰胺诱导表达所需时间更短。高浓度的2-氯苯磺酰胺影响芥菜种子发芽及生长发育,并且明显抑制IN2-2启动子的表达活性。4℃低温胁迫诱导IN2-2启动子在幼苗叶片中表达,IN2-2启动子轻微受150mmol·L-1Na Cl胁迫表达,但不受重金属Gu~(2+)离子的诱导表达。
IN2-2 :: GUS transgenic Brassica juncea was treated with acetaminophen and 2-chlorobenzenesulfonamide with different concentrations (50, 100 and 200 mg · L -1) of herbicide-safener respectively. The results showed that after acetanilide and 2-chlorobenzenesulfonamide treatment, the IN2-2 promoter was expressed in calyx, petal, stamens and pollen of mustard, leaf and floral organs, but not in ovule. 100 mg · L-1 acetanilide and 50 mg · L-12-chlorobenzenesulfonamide are suitable for regulating the expression of the IN2-2 promoter in Brassica juncea, with acetanilide being less time-consuming than 2-chlorobenzenesulfonamide to induce expression. High concentrations of 2-chlorobenzenesulfonamide affect the germination and growth of B. juncea seeds and significantly inhibit the expression activity of the IN2-2 promoter. The IN2-2 promoter was induced to express in seedling leaves at 4 ℃ under low temperature stress. IN2-2 promoter was slightly expressed under the stress of 150 mmol·L-1 NaCl, but was not induced by heavy metal ions.