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从野生毛葡萄组织中克隆了9个质膜水通道蛋白基因(VhPIP)的c DNA全长序列,并研究了其生物信息学特征及组织特异性表达。氨基酸序列分析表明,9个VhPIP分为PIP1和PIP2两类;均含有MIP蛋白家族保守的信号序列SGGHINPAVT和两个NPA(Asn-Pro-Ala)单元,均为稳定性蛋白、疏水性蛋白,且每个VhPIP均含有6个跨膜区。实时荧光定量RT-PCR分析表明,这9个VhPIP在抗旱的毛葡萄‘花溪–9’根、茎、叶中均有表达,其中VhPIP1;1、VhPIP1;2、VhPIP1;4、VhPIP2;1、VhPIP2;2和VhPIP2;4在其根中的表达量最多,可能与根部水分运输的调节密切相关;VhPIP1;5和VhPIP2;3在其叶中的表达量最多,可能主要参与叶片的水分运输,与叶片的蒸腾作用密切相关;VhPIP1;3在根、茎和叶中的表达量无显著性差异;相对于其他基因的表达,VhPIP1;1和VhPIP2;4在根中表达量最高,这两个基因可能在水分运输过程中起较大作用。
Nine full-length cDNA sequences encoding the plasma membrane aquaporin (VhPIP) genes were cloned from wild grapevine tissue and characterized by bioinformatics and tissue-specific expression. Amino acid sequence analysis showed that 9 VhPIPs were classified as PIP1 and PIP2. They all contained the conserved signal sequence SGGHINPAVT and two NPA (Asn-Pro-Ala) units of the MIP family, which were all stable proteins and hydrophobic proteins. Each VhPIP contains six transmembrane regions. The results of real-time RT-PCR showed that the nine VhPIPs were expressed in roots, stems and leaves of ’Huaxi-9’ with drought resistance, including VhPIP1, VhPIP1, VhPIP1, VhPIP2, VhPIP2; 2 and VhPIP2; 4 expressed most in their roots, which may be closely related to the regulation of root water transport. VhPIP1; 5 and VhPIP2; 3 had the highest expression in leaves and probably involved in leaf water transport, Which was closely related to the transpiration of leaves; VhPIP1; 3 had no significant difference in root, stem and leaf expression; VhPIP1; 1 and VhPIP2; 4 had the highest expression in the roots compared with other genes, Genes may play a larger role in the transport of water.