甲基还原青蒿素(MDHA)是青蒿素的衍生物,经药理及临床试验证实其抗疟疗效优于青蒿素,为了开展体内过程及药代动力学研究,使给药方案更加科学合理,急需建立生物样品的测定方法。本文报道血浆中MDHA的薄层扫描定量法。本法特异性较高,检出下限为0.1μg,CV低于13.0％,血浆回收率达83％以上。 Methyl-reducing artemisinin (MDHA) is a derivative of artemisinin. Its pharmacological and clinical trials have demonstrated that its antimalarial efficacy is better than artemisinin. In order to carry out in vivo processes and pharmacokinetics studies, the dosing regimen is more scientific. Reasonable, it is urgent to establish a method for the determination of biological samples. In this paper, thin-layer quantification of plasma MDHA is reported. The specificity of this method is higher, the detection limit is 0.1μg, CV is lower than 13.0%, and the plasma recovery rate is more than 83%.