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目的:建立不同产地金盏银盘药材的高效液相色谱指纹图谱,并同时测定金丝桃苷、异槲皮苷、鬼针聚炔苷和紫云英苷4种主要成分的含量。方法:采用反相高效液相色谱法,选用Hypersil ODS C18(250 mm×4.6 mm,5μm)色谱柱,乙腈-3%冰醋酸为流动相梯度洗脱,采用药典委员会颁布的中药色谱指纹图谱相似度评价系统(2004 A)评价图谱的相似性,同时测定药材中4种主要成分的含量。结果:建立了不同产地金盏银盘药材的HPLC指纹图谱,方法学符合指纹图谱要求,不同产地药材相似度差异不大,但4种主要成分含量差异较大。结论:该方法稳定、准确、可靠,指纹图谱结合含量测定为金盏银盘药材质控标准的制定提供了科学依据。
OBJECTIVE: To establish HPLC fingerprint of Radix Glabrae Radix in different areas and to determine the contents of 4 main components of hyperoside, isoquercitrin, spermatophosphamide and astragalin. Methods: The chromatographic fingerprint of Chinese traditional medicine was obtained by HPLC using Hypersil ODS C18 (250 mm × 4.6 mm, 5 μm) column and acetonitrile-3% glacial acetic acid as mobile phase. Degree Evaluation System (2004 A) Evaluation of the similarity of the spectrum, and determination of the content of the four main components of medicinal materials. Results: The HPLC fingerprints of Gynostemma officinale from different areas were established. The method was consistent with the fingerprinting requirements. The similarities of medicinal materials from different areas were not obvious, but the contents of the four main components differed greatly. CONCLUSION: The method is stable, accurate and reliable, and the determination of the combination of fingerprint and fingerprint provides a scientific basis for the quality control standard of Gynostemma officinalis.