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本文报道用反相高效液相色谱法测定血清和尿液中洛美沙星浓度，固定相用Ｃ＿（１８）柱，流动相为甲醇／０．００８Ｍ磷酸盐缓冲液／０．５Ｍ四丁基溴化铵（２５：７５：４，Ｖ／Ｖ／Ｖ）．荧光检测激发波长２８０ｎｍ，发射波长４５５ｎｍ，以外标法定量。洛美沙星的最低检出限为０．０６μｇ／ｍｌ，线性范围为０．１２５－８．μｇ／ｍｌ．线性关系良好（ｒ＝０．９９９９），日内变异系数２．８７％，日间变异系数２．９４％，回收率７５．８％～８０．９％。
In this paper, the concentrations of lomefloxacin in serum and urine were determined by reversed-phase high performance liquid chromatography. The stationary phase was eluted with C 18 column and the mobile phase was methanol / 0.008 M phosphate buffer / 0.5 M tetrabutyl bromide Ammonium chloride (25: 75: 4, V / V / V). Fluorescence detection excitation wavelength 280nm, emission wavelength 455nm, external standard method. The minimum detectable limit of lomefloxacin was 0.06 μg / ml with a linear range of 0.125-8. μg / ml. The linearity was good (r = 0.9999), the intra-day coefficient of variation was 2.87%, the intra-day coefficient of variation was 2.94% and the recovery was 75.8% -80.9%.