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目的:比较研究人骨髓源和胎盘源间充质干细胞介导的T细胞增殖抑制作用机制。方法:应用流式细胞术(FCM)分别检测B7H4和PDL1在人骨髓源间充质干细胞(HBMSCs)和胎盘源间充质干细胞(HPMSCs)上的表达;应用抗体阻断试验分析B7H4、PDL1分别在HBMSCs和HPMSCs对T细胞增殖及周期影响中的作用。结果:HBMSCs上高表达免疫负性调控分子B7H4,而HPMSCs上高表达免疫负性调控分子PDL1。分别应用B7H4mAb和PDL1mAb阻断,可使HBMSCs和HPMSCs对PHA激发的T细胞增殖抑制作用明显减弱;下调T细胞周期中G0/G1期细胞数量,上调S期细胞数量,明显减弱HBMSCs和HPMSCs对T细胞周期的影响。结论:HBMSCs和HPMSCs可通过表达不同的免疫负性调控分子介导T细胞的增殖抑制作用。
OBJECTIVE: To compare the inhibitory mechanism of human bone marrow-derived mesenchymal stem cell-derived T cell proliferation with human bone marrow. Methods: The expression of B7H4 and PDL1 on human bone marrow mesenchymal stem cells (HBMSCs) and placental derived mesenchymal stem cells (HPMSCs) were detected by flow cytometry (FCM) respectively. Antibody blocking assay was used to analyze the expression of B7H4 and PDL1 Effect of HBMSCs and HPMSCs on proliferation and cycle of T cells. Results: HBMSCs expressed high level of negative immunoregulatory molecule B7H4, whereas HPMSCs showed high expression of negative immunoregulatory molecule PDL1. B7H4mAb and PDL1mAb respectively blocked HBMSCs and HPMSCs significantly inhibited the proliferation of PHA-stimulated T cells; down-regulated the number of cells in G0 / G1 phase and up-regulated the number of S phase cells in T-cell cycle, Effect of cell cycle. Conclusion: HBMSCs and HPMSCs can mediate the inhibition of T cell proliferation by expressing different immunoregulatory molecules.