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目的研究硒能否拮抗铅上调SH-SY5Y和PC12中β-淀粉样蛋白40和β-淀粉样蛋白前体蛋白表达。方法用醋酸铅和硒蛋氨酸联合染毒SH-SY5Y和PC12细胞,CCK8试剂盒测定细胞存活率,蛋白印迹法和酶联免疫法测定β-淀粉样蛋白前体蛋白和β-淀粉样蛋白40表达。结果单独染铅48 h后,SH-SY5Y和PC12两种细胞中β-淀粉样蛋白40和β-淀粉样蛋白前体蛋白表达均增加,且随着铅剂量的增加表达量增加;与高剂量染铅组相比,铅硒联合作用组β-淀粉样蛋白40和β-淀粉样蛋白前体蛋白表达下降,且随着硒剂量的增加表达量减少。结论硒能拮抗铅上调β-淀粉样蛋白表达的作用。
Objective To study whether selenium can antagonize lead up-regulation of β-amyloid 40 and β-amyloid precursor protein expression in SH-SY5Y and PC12. Methods SH-SY5Y and PC12 cells were treated with lead acetate and selenomethionine. Cell viability was determined by CCK8 kit. The expression of β-amyloid precursor protein and β-amyloid protein 40 were detected by Western blotting and enzyme-linked immunosorbent assay. Results The expression of β-amyloid protein 40 and β-amyloid precursor protein increased in both SH-SY5Y and PC12 cells after lead exposure for 48 h, and increased with the increase of lead dose. Compared with high dose Compared with the lead-exposed group, the expression of β-amyloid 40 and β-amyloid precursor protein decreased with the combination of lead and selenium, and decreased with the increase of selenium dosage. Conclusion Selenium can antagonize lead up-regulation of β-amyloid protein expression.