论文部分内容阅读
目的探索Sertoli细胞对淋巴细胞的作用,为人工构建免疫豁免等提供思路。方法酶解法分离培养幼龄大鼠睾丸的Sertoli细胞;用丝裂原ConA、LPS分别刺激T、B淋巴细胞活化;用MTT法测定Sertoli细胞上清作用过的淋巴细胞增值活力;用AnnexinV/PI法测定经Sertoli细胞培养上清作用后淋巴细胞的凋亡率。结果 Sertoli细胞培养上清明显抑制ConA(MTT测定OD值阳性/对照:0.22/0.11)和LPS(MTT测定OD值阳性/对照:0.19/0.09)对淋巴细胞的促增殖作用,也可诱导这两种淋巴细胞凋亡率上升(34.5Vs29.9与48.6Vs33.9)。结论 Sertoli细胞培养上清对丝裂原激活的T、B细胞具有增殖抑制和凋亡诱导作用。
Objective To explore the effect of Sertoli cells on lymphocytes and provide ideas for artificial immunization. Methods Sertoli cells were isolated from the testes of young rats by enzymolysis method. T and B lymphocytes were stimulated with ConA and LPS respectively. The viability of T lymphocytes was measured by MTT assay. Method to determine the apoptosis rate of lymphocytes after the effect of Sertoli cell culture supernatant. Results The proliferation of Sertoli cell culture supernatant obviously inhibited the proliferation of lymphocytes induced by ConA (OD value of MTT assay / control: 0.22 / 0.11) and LPS (OD of MTT assay assay / control: 0.19 / 0.09) The rate of apoptosis of lymphocytes increased (34.5Vs29.9 and 48.6Vs33.9). Conclusion Sertoli cell culture supernatant can inhibit proliferation and induce apoptosis of mitogen-activated T and B cells.