目的:建立复方奥硝唑大黄口腔膜中奥硝唑的含量测定方法。方法:采用高效液相色谱法,VP-ODS C18(4.6mm×150mm,5μm),柱温为40℃,甲醇-水(3∶7)为流动相,流速为1.0mL·min-1,检测波长为318nm。结果:奥硝唑在15～500mg·L-1范围内呈良好线性关系(r=0.9998)。平均加样回收率为100.23%,RSD为0.26%(n=6)。结论:本法快速、简便、精确,可作为复方奥硝唑大黄口腔膜中奥硝唑的定量分析方法。 Objective: To establish a method for the determination of ornidazole in compound ornidazole rhubarb oral films. Methods: VP-ODS C18 (4.6mm × 150mm, 5μm) was used in this study. The column temperature was 40 ℃, methanol-water (3: 7) was used as the mobile phase and the flow rate was 1.0mL · min-1. The wavelength is 318nm. Results: Ornidazole showed a good linearity (r = 0.9998) in the range of 15 ~ 500 mg · L-1. The average recovery was 100.23% with a RSD of 0.26% (n = 6). Conclusion: This method is rapid, simple and accurate and can be used as a quantitative method for the determination of ornidazole in the oral orofacial ornidazole rhubarb compound.