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为阐明投射至骶髓后连合核的盆内脏初级传入中是否含有传递伤害性信息成分,本研究综合运用特异性标记初级传入C纤维的BSI-B4-HRP跨节追踪技术,神经干局部涂抹C纤维毒素CaPsaicin并结合SP免疫组化方法,研究了猫投射至骶髓后连合核的盆神经初级传入纤维中是否含有传递伤害性刺激的成分;同时观察了秋水仙素处理的骶2后根节内BSI-B4标记的初级传入神经元与SP免疫阳性神经元的关系。结果如下:(1)向盆神经注入BSI-B4-HRP,骶1~3后根节内出现平均直径34μm的标记细胞,后连合核内出现密集的标记终末,电镜下证明通过Lissauer氏束进入脊髓内的标记纤维均为无髓纤维;(2)对盆神经进行局部Capsaicin处理,引起后连合核内的SP免疫阳性纤维和终末明显减少;(3)骶2后根节内BSI-B4-FITC标记细胞有17%同时呈SP免疫阳性;(4)骶2后根节内BSI-B4-HRP标记的盆内脏初级传人神经元的39%同时呈SP免疫阳性。本研究结果在形态学上证实了骶髓后连合核接受盆腔内脏伤害性信息的传入,它可能是中继和整合盆内脏伤害性信息的低级中枢。
In order to elucidate whether the primary afferent information of pelvic viscera projected to the sacral spinal dorsal commissure contains transmission-injurious information components, BSI-B4-HRP cross-sectional tracing technique, which specifically labeled primary afferent fibers, Topical application of C-fiber toxin CaPsaicin combined with SP immunohistochemistry to study the cat’s projection into the sacral spinal dorsal commissure nucleus of the primary afferent nerve fibers containing transmission of nociceptive stimuli; also observed the colchicine-treated Relationship between Primary BSI-B4-labeled Primary Neurons and SP-immunoreactive Neurons in Sacral 2-Dorsal Root Ganglion. The results were as follows: (1) BSI-B4-HRP was injected into the pelvic nerve, and labeled cells with an average diameter of 34μm appeared in the root segments of the sacral 1 to 3, and dense labeled terminals appeared in the posterior commissural nucleus. Electron microscopy showed that Lissauer’s (2) local Capsaicin treatment of the pelvic nerve, resulting in posterior commissure nucleus of SP immunoreactive fibers and terminals were significantly reduced; (3) sacral 2 root in the dorsal root ganglia 17% of BSI-B4-FITC-labeled cells were also SP immunopositive; (4) 39% of BSI-B4-HRP-labeled primary pelvic visceral neurons in sacral 2 DRG were also SP immunopositive. The results of this study confirm morphologically the introduction of pelvic visceral nociceptive information into the posterior commissural nucleus of the sacral canal, which may be the low-level backbone for relaying and integrating pelvic visceral nociceptive information.