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目的通过对水产品、外环境和腹泻便中副溶血弧菌的分离鉴定,掌握副溶血弧菌的污染分布状况,有效预警食源性疾病的发生,同时对分离出的副溶血弧菌进行耐药性分析,为临床用药提供科学依据。方法水产品按GB 4789.7-2013检验,外环境参照海水中副溶血弧菌检验方法的优化第一法,腹泻便检验依据WS 271-2007附录B,分离鉴定出副溶血弧菌,并对分离出的副溶血弧菌进行耐药性试验。结果水产品、外环境和腹泻便中副溶血弧菌检出率分别为21.72%、1.61%和12.38%,其中水产品中鱼、虾、蟹、贝和软体动物的检出率分别为16.56%、27.27%、20.41%、29.46%和10.00%。外环境中水体、泥沙、水生植物和水生动物检出率分别为2.16%、1.71%、0.76%和1.33%。腹泻便中检出率为12.38%。结论山海关区腹泻便中副溶血弧菌耐药日趋严重,推荐环丙沙星和左氧氟沙星等喹诺酮类抗生素作为治疗副溶血弧菌感染的首选药物。
OBJECTIVE To isolate and identify Vibrio parahaemolyticus in aquatic products, external environment and diarrhea, to master the pollution distribution of Vibrio parahaemolyticus and to effectively prevent the occurrence of foodborne diseases. Meanwhile, Vibrio parahaemolyticus Medicinal analysis for clinical use to provide a scientific basis. Methods Aquatic products according to GB 4789.7-2013 test, the external environment reference seawater Vibrio parahaemolyticus test method optimization first method, diarrhea will be tested according to WS 271-2007 Appendix B, isolated and identified Vibrio parahaemolyticus, and isolated Vibrio parahaemolyticus drug resistance test. Results The detection rates of V. parahaemolyticus in aquatic products, external environment and diarrhea were 21.72%, 1.61% and 12.38% respectively. The detection rates of fish, shrimp, crab, shellfish and molluscs in aquatic products were 16.56% , 27.27%, 20.41%, 29.46% and 10.00% respectively. The detection rates of water, sediment, aquatic plants and aquatic animals in the outer environment were 2.16%, 1.71%, 0.76% and 1.33% respectively. Diarrhea in the detection rate was 12.38%. Conclusions The drug resistance of Vibrio parahaemolyticus is becoming more and more serious in the diarrhea in Shanhaiguan district. Quinolone antibiotics such as ciprofloxacin and levofloxacin are recommended as the drug of choice for the treatment of Vibrio parahaemolyticus infection.