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目的通过检测乙肝患者血清中乙肝病毒外膜大蛋白(HBV-LP)、HBV、DNA、以及乙肝两对半(HBVM),探讨HBV-LP与HBVDNA浓度的相关性。方法共检测354例乙肝患者血清标本,HBV-LP、乙肝前S1蛋白(Pre-SlAg)以及乙肝两对半采用酶联免疫方法进行检测,采用实时荧光定量PCR方法检测HBVDNA。结果(1)在290份HBVDNA阳性标本中HBV-LP的阳性率(88.62%)明显高于乙肝病毒前S1的阳性率(48.97%),两者之间差异有显著性(P<0.05);(2)HBV-LP吸光度值与HBVDNA拷贝数呈正相关性,相关系数r=0.939;(3)在HBeAg阳性和阴性标本中,Pre-SlAg的检出率则明显低于HBV-LP和HBVDNA。结论本研究结果显示HBV-LP与HBVDNA有良好的正相关性,HBV-LP检出与HBVDNA的符合率高于Pre-SlAg,HBV-LP检测可以用于反映乙肝患者病毒复制的血清免疫学指标。
Objective To explore the relationship between HBV-LP and HBVDNA by detecting HBV-LP, HBV, DNA and HBVM in serum of hepatitis B patients. Methods A total of 354 serum samples from patients with hepatitis B, HBV-LP, Pre-S1Ag and HBV DNA were detected by enzyme-linked immunosorbent assay (ELISA). HBVDNA was detected by real-time fluorescence quantitative PCR. Results (1) The positive rate of HBV - LP in 88 HBVDNA positive specimens (88.62%) was significantly higher than that of HBV pre - S1 specimens (48.97%). There was a significant difference between the two (P <0.05). (2) There was a positive correlation between HBV-LP absorbance and HBVDNA copy number (r = 0.939). (3) The detection rate of Pre-S1Ag in HBeAg positive and negative samples was significantly lower than that of HBV-LP and HBVDNA. Conclusion The results of this study showed that there was a positive correlation between HBV-LP and HBVDNA. The coincidence rate of HBV-LP and HBVDNA was higher than that of Pre-S1Ag. HBV-LP could be used to reflect the serum immunological index of HBV replication .