论文部分内容阅读
目的：观察３ 种厌氧菌荚膜对人骨髓长时间培养中破骨细胞形成和功能活化的影响。方法：采用合法堕胎５ 个月胎儿长干骨骨髓单个核细胞，在 Ｇ Ｍ－ Ｃ Ｓ Ｆ、马血清和荚膜存在下培养３ 周，观察破骨细胞的形成。结果：在所有培养中由单个核细胞融合形成的多核细胞多数具有破骨细胞特征：多核、细胞膜呈皱褶状、抗酒石酸盐酸性磷酸酶染色阳性，在牙本质片上可形成吸收陷窝等。牙龈卟啉菌荚膜组和中间型普里沃氏菌荚膜组中，抗酒石酸盐酸性磷酸酶染色阳性的多核和单个核细胞数和牙本质片上形成的吸收陷窝明显增多。牙髓卟啉菌荚膜组与对照组差异不明显。结论：厌氧菌荚膜可能参与了牙周病、尖周病后期的骨组织吸收。
Objective: To observe the effects of three kinds of anaerobic capsular capsules on the formation and functional activation of osteoclasts in long-term culture of human bone marrow. Methods: Marrow bone marrow mononuclear cells from fetal fetuses aged 5 months with legal abortion were cultured in G M-CSF, horse serum and capsule for 3 weeks to observe the formation of osteoclasts. Results: The majority of multinucleate cells formed by fusion of mononuclear cells in all cultures had osteoclast characteristics: multinuclear, follicular cell membrane, positive staining with anti-tartaric acid phosphatase, and absorption lacuna in dentin. In the Porphyromonas gingivalis capsule group and the intermediate Prunella capsulae group, the numbers of multinucleated and mononuclear cells positive for tartrate-resistant acid phosphatase staining and the absorption lacuna formed on the dentin sheet were significantly increased. There was no significant difference between Porphyromonas capsule group and control group. Conclusion: The anaerobic capsule may be involved in periodontal disease and bone resorption in the late period of acuminatum.