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采用原癌基因Ｂｃｌ－２、Ｐ２１及抑癌基因Ｐ５３、Ｒｂ蛋白４种抗体，用ＡＢＣ免疫组织化学方法对４０例脑星形胶质瘤组织作免疫组化染色．结果发现：Ｂｃｌ－２及Ｐ５３基因表达在Ⅰ～Ⅳ级胶质瘤中阳性率有显著性差异，而且染色强度也有明显差异．Ｐ２１、Ｒｂ基因表达在Ⅰ～Ⅳ级胶质瘤中呈上升趋势，但仅在Ⅰ～Ⅲ级、Ⅰ～Ⅳ级之间有明显差异．可以认为：Ｂｃｌ－２、Ｐ５３、Ｐ２１、Ｒｂ在胶质瘤的发生中发挥一定的作用，尤其是Ｂｃｌ－２、Ｐ５３基因，这些基因蛋白的表达与胶质瘤恶性程度有关．测定胶质瘤中的Ｂｃｌ－２、Ｐ５３、Ｐ２１、Ｒｂ蛋白对于胶质瘤的诊断、鉴别诊断以及判断肿瘤的恶性程度与预后均有一定意义．
The immunohistochemical staining of 40 cases of brain astrocytoma was performed with ABC immunohistochemistry using four antibodies against oncogene Bcl-2, P21 and tumor suppressor genes P53 and Rb. The results showed that the expression of Bcl-2 and P53 gene in grade I-IV gliomas was significantly different, and the staining intensity was also significantly different. The expression of P21 and Rb genes showed an upward trend in grade I-IV gliomas, but there were significant differences between grades I-III and grades I-IV. It can be considered that Bcl-2, P53, P21, and Rb play a role in the development of gliomas, especially the Bcl-2 and P53 genes. The expression of these genes is related to the degree of malignancy of gliomas. The determination of Bcl-2, P53, P21 and Rb proteins in gliomas is of significance in the diagnosis and differential diagnosis of gliomas and in judging the malignancy and prognosis of gliomas.