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目的通过静脉注射氟尿嘧啶建立稳定有效的化疗性静脉炎小鼠模型。方法 42只昆明小鼠按照给药浓度随机分为7.5 mg/mL、10.0 mg/mL、12.5 mg/mL,15.0 mg/mL、17.5 mg/mL和22.5 mg/mL共6个实验组和1个对照组。实验组小鼠分别从左侧鼠尾静脉注射0.4 mL不同浓度的氟尿嘧啶溶液,对照组注射0.4mL的生理盐水。给药后第3 d对化疗性静脉炎表现进行分级评价后处死。制作石蜡切片并进行镜下分级。结果随注射氟尿嘧啶剂量的增加,小鼠静脉炎发生率也逐渐增高,350 mg/kg(17.5 mg/mL组)以上剂量组动物出现中毒死亡。300 mg/kg(15.0 mg/mL)剂量组化疗性静脉炎发生率达100%,无动物死亡,出现典型的静脉炎症状,对照组未出现类似变化。结论通过小鼠尾静脉注射300 mg/kg～350 mg/kg剂量范围的氟尿嘧啶可成功建立稳定有效的化疗性静脉炎小鼠模型。
Objective To establish a stable and effective chemotherapy phlebitis mouse model by intravenous fluorouracil. Methods Forty-two Kunming mice were randomly divided into 6 experimental groups (7.5 mg / mL, 10.0 mg / mL, 12.5 mg / mL, 15.0 mg / mL, 17.5 mg / mL and 22.5 mg / mL) Control group. Experimental mice were injected 0.4 mL of fluorouracil solution of different concentrations from the left mouse tail vein and 0.4 mL of normal saline into the control group respectively. On the 3rd day after administration, the performance of chemotherapy phlebitis was evaluated by grading and sacrificed. Paraffin sections were made and microscopically graded. Results The incidence of phlebitis in mice increased gradually with the increase of the dose of fluorouracil injection. The animals in the doses above 350 mg / kg (17.5 mg / mL) died. The incidence of chemotherapy phlebitis in the 300 mg / kg (15.0 mg / mL) dose group was 100%, with no animals dead, typical phlebitis symptoms and no similar changes in the control group. Conclusion A stable and effective chemotherapy phlebitis mouse model can be established successfully by injecting 5-FU with 300 mg / kg ~ 350 mg / kg in the tail vein of mice.