论文部分内容阅读
目的探讨人参皂甙Rg1、Rb1对糖尿病肾病大鼠肾组织TGF-β1mRNA及蛋白表达的影响。方法采用腹腔内一次性注射STZ 55 mg/kg复制糖尿病大鼠模型。各组分别给予相应药物灌胃治疗;每周测体重,于第4、8、12周末用考马斯亮蓝法测24 h尿蛋白。12周后检测BUN、Scr、TG,计算肾重体重比,肾组织进行HE、Mallory染色,观察大鼠肾脏病理学变化。用免疫组化、原位杂交方法分别检测肾组织TGF-β1mRNA及其蛋白的表达。结果Rg1、Rb1组大鼠体重各周均高于模型组,但没有统计学差异。Rb1组大鼠肾重体重比具有极显著性差异(P<0.01),Rg1大剂量组、Rg1小剂量组有显著性差异(P<0.05)。与模型组比较,Rg1大剂量组、Rg1小剂量组及Rb1组大鼠在第4周、第8周,可以降低24 h尿蛋白含量,有极显著性差异(P<0.01),第12周,Rg1大剂量组、Rg1小剂量组及Rb1组有显著性差异(P<0.05)。血液生化方面与模型组比较,Rg1大剂量组、Rg1小剂量组及Rb1组可以降低大鼠BUN,均有极显著性差异(P<0.01);可以降低Scr,Rg1大剂量组及Rb1组有极显著性差异(P<0.01),而Rg1小剂量组有显著性差异(P<0.05);同时Rg1、Rb1可以减轻肾脏病理学改变。与模型组比较,Rg1小剂量组、Rg1大剂量组及Rb1组可以减少肾组织TGF-β1蛋白的表达,均有极显著减少(P<0.01);Rg1小剂量组、Rg1大剂量组及Rb1组TGF-β1mRNA表达,具有有极显著性差异(P<0.01)。结论Rg1、Rb1通过抑制DN大鼠肾组织TGF-β1mRNA及蛋白表达,进而保护DN大鼠肾脏功能,这可能是Rg1、Rb1防治糖尿病肾病进展的机制之一。
Objective To investigate the effect of ginsenoside Rg1 and Rb1 on the expression of TGF-β1 mRNA and protein in renal tissue of rats with diabetic nephropathy. Methods Diabetic rats were intraperitoneally injected with STZ 55 mg/kg. Each group was given a corresponding drug gavage treatment; body weight was measured weekly, 24 h urinary protein was measured by Coomassie brilliant blue method on the 4th, 8th and 12th weekends. BUN, Scr, and TG were measured after 12 weeks. The ratio of kidney weight to body weight was calculated. Renal tissue was stained with HE and Mallory to observe the pathological changes of the kidney. Immunohistochemistry and in situ hybridization were used to detect the expression of TGF-β1 mRNA and protein in renal tissue. Results The weights of rats in Rg1 and Rb1 groups were higher than those in the model group at each week, but there was no statistical difference. The Rb1 group had significant difference in kidney weight ratio (P<0.01). There was a significant difference in Rg1 high dose group and Rg1 low dose group (P<0.05). Compared with the model group, the Rg1 high-dose group, the Rg1 low-dose group and the Rb1 group could reduce the 24-hour urinary protein level at the 4th and 8th week, with extremely significant difference (P<0.01). There was significant difference between Rg1 high dose group, Rg1 low dose group and Rb1 group (P<0.05). Compared with the model group in blood biochemistry, Rg1 high-dose group, Rg1 low-dose group and Rb1 group can reduce BUN in rats, there is a very significant difference (P <0.01); Scr, Rg1 high-dose group and Rb1 group can be reduced There was a significant difference (P<0.01), while there was a significant difference in the low-dose Rg1 group (P<0.05). At the same time, Rg1 and Rb1 could reduce the renal pathological changes. Compared with the model group, Rg1 low-dose group, Rg1 high-dose group and Rb1 group can reduce the expression of TGF-β1 protein in renal tissue, all significantly reduced (P <0.01); Rg1 low-dose group, Rg1 high-dose group and Rb1 The expression of TGF-β1 mRNA in the group had a very significant difference (P<0.01). Conclusion Rg1 and Rb1 protect kidney function of DN rats by inhibiting the expression of TGF-β1 mRNA and protein in renal tissue of DN rats, which may be one of the mechanisms of Rg1 and Rb1 in preventing and treating diabetic nephropathy.