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目的:鉴定幽门螺杆菌(Hp)与宿主细胞人类胃上皮细胞(AGS)作用后差异蛋白的改变。方法:应用双向电泳分离菌株26695与AGS细胞相互作用前后的蛋白,应用Image Master 2Dv3.1软件对蛋白图谱进行比较,对目的蛋白进行基质辅助激光解析及电离飞行时间质谱分析,应用Mascot软件进行蛋白搜库。结果:共监测到5个差异蛋白斑点,经质谱分析鉴定为2种蛋白,分别是热休克蛋白60和烷基过氧化还原酶。结论:烷基过氧化还原酶可能进入AGS细胞或结合于AGS细胞膜上,有助于明确Hp与宿主细胞相互作用后的致病过程。
Objective: To identify the differential proteins after Helicobacter pylori (Hp) and host cell human gastric epithelial cells (AGS). Methods: Proteins of strain 26695 before and after interaction with AGS cells were separated by two-dimensional electrophoresis. The protein profiles were compared with Image Master 2Dv3.1 software. Matrix-assisted laser analysis and ionization time-of-flight mass spectrometry analysis were performed on the target proteins. Search library. Results: A total of 5 spots of differential proteins were detected and identified as two proteins by mass spectrometry analysis, namely, heat shock protein 60 and alkyl peroxidase. CONCLUSION: Alkyl peroxidase may enter AGS cells or bind to AGS cell membrane, which helps to clarify the pathogenic process after Hp interacts with host cells.