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目的用染色体8p微卫星的杂合性缺失(LOH)作为肿瘤的遗传性标记,对同一肝细胞癌结节内部不同肿瘤细胞群是否有克隆差异进行研究。方法在10例肝细胞癌患者,同一肿瘤结节内部不同颜色质地的部分作为不同的细胞群,手工辅助显微切割获取较纯的肿瘤细胞,抽提肿瘤细胞DNA;选取5个染色体8p微卫星位点D8S277、D8S254、D8S258、D8S298、D8S1771,进行微卫星LOH的检测。结果在10例肝细胞癌患者,7例检测出8p微卫星的LOH,3例未检测出8p微卫星的LOH。6例患者在同一肿瘤结节的内部不同肿瘤细胞群之间出现微卫星位点LOH类型的差异,1例患者3个肿瘤细胞群的LOH类型相同。结论在同一肝细胞癌结节的内部,不同的肿瘤细胞群之间存在克隆差异,这种差异说明不同的肿瘤细胞群可能有不同的克隆来源,从而为观察同一肿瘤内不同肿瘤细胞群的生物学特性提供依据。
OBJECTIVE: To investigate whether there are clonal differences in different tumor cell populations in the same hepatocellular carcinoma nodule using the heterozygosity deletion (LOH) of microsatellite chromosome 8p as a genetic marker of tumor. Methods Ten patients with hepatocellular carcinoma (HCC) were divided into different cell masses with different color texture in the same tumor nodule, and the pure tumor cells were obtained by hand assisted microdissection. The DNA of tumor cells was extracted. Five microsatellite chromosomes Site D8S277, D8S254, D8S258, D8S298, D8S1771, microsatellite LOH detection. Results In 10 patients with hepatocellular carcinoma, 7 cases detected 8p microsatellite LOH and 3 detected 8p microsatellite LOH. Six patients showed differences in the LOH type of microsatellite loci between different tumor cell populations within the same tumor nodules, and one patient had the same LOH type in the three tumor cell populations. Conclusion Cloning differences exist between different tumor cell populations within the same hepatocellular carcinoma nodules. This difference indicates that different tumor cell populations may have different cloning sources, so as to observe the biological characteristics of different tumor cell populations in the same tumor Learn to provide basis for the characteristics.