应用流式细胞仪(FCM)和长春新碱(VS)阻断细胞有丝分裂的方法分析三种不同阶段生长的非正倍体小鼠腹水肿瘤细胞,计算其细胞增殖率。实验用三种非正倍体小鼠腹水瘤JB-I,LIA_2和PNJ,腹腔接种5×10~6肿瘤细胞体内传代,潜伏期为8～10天。在接种后不同时间,注射细胞有丝分裂阻断剂VS(5 mg/kg),注射后在0、2、4、6和8小时分别抽腹水50μl,含有肿瘤细胞2-5×10~6。用FCM分析DNA含量,并在镜下计数有丝分裂细胞百 Using flow cytometry (FCM) and vincristine (VS) to block cell mitosis, three different stages of non-euploid mouse ascites tumor cells were analyzed and the cell proliferation rate was calculated. Three types of non-euploid mouse ascites tumors JB-I, LIA_2 and PNJ were inoculated intraperitoneally with 5×10 6 tumor cells in vivo for a period of 8 to 10 days. At different times after inoculation, the cells were injected with mitotic blocking agent VS (5 mg/kg). After injection, 50 μl of ascitic fluid was pumped at 0, 2, 4, 6 and 8 hours and contained 2-5×10 6 tumor cells. Analysis of DNA content by FCM and counting of mitotic cells 100