论文部分内容阅读
目的探讨大肠埃希菌感染对雄性小鼠生殖细胞凋亡和P53蛋白表达的影响,从分子水平阐明清利生精丸治疗大肠埃希菌感染所致男性不育的机制。方法 50只雄性小鼠,经膀胱注射大肠埃希菌,建立动物感染模型,观察15d后随机分为5组:感染组(不治疗)、清利生精丸大、中、小等3种浓度治疗组(药物浓度分别为22.5、13.5和4.50g/ml)、呋喃坦啶治疗组,分别编码为MN、MTa、MTb、MTc、MTd组,另取10只小鼠膀胱注射生理盐水作为正常对照组(编码为CT)。各治疗组连续灌胃药液10d,应用流式细胞术检测小鼠睾丸生殖细胞凋亡率,免疫组化技术测定生殖细胞P53蛋白表达情况。结果各组小鼠均有一定的睾丸生殖细胞凋亡率,其中MN组生殖细胞凋亡率57.44%,CT组28.54%,MTa组30.11%,MTb组28.59%,差异有统计学意义(P<0.01);MN组生殖组细胞凋亡率与MTc(46.54%)和MTd组(43.41%)比较差异无统计学意义(P>0.05)。MN、CT、清利生精丸各治疗组和MTd组表达P53蛋白睾丸生殖细胞百分比差异有统计学意义(P<0.01)。结论大肠埃希菌感染小鼠后,可导致小鼠睾丸生殖细胞P53蛋白的表达上升,生殖细胞凋亡增加。清利生精丸通过下调P53蛋白,降低生殖细胞的凋亡率,提高小鼠生殖能力。因此,清利生精丸是治疗大肠埃希菌感染性不育的有效的药物之一。
Objective To investigate the effects of Escherichia coli infection on germ cell apoptosis and P53 protein expression in male mice and to elucidate the mechanism of Qingliang Spermatogenic Pill in treating male infertility caused by Escherichia coli infection at the molecular level. Methods Fifty male mice were injected with Escherichia coli through the bladder to establish an animal model of infection. After 15 days, the mice were randomly divided into 5 groups: infection group (no treatment), Qingliangsheng pill (large, medium and small) (22.5, 13.5 and 4.50 g / ml respectively), and furadanthin treatment group, which were respectively coded as MN, MTa, MTb, MTc and MTd groups, and another 10 mice were injected with normal saline into the bladder as normal control group Encoded as CT). Each treatment group was administered with liquid for 10 days. The apoptosis rate of testicular germ cells was detected by flow cytometry. The expression of P53 protein in germ cells was detected by immunohistochemistry. Results The apoptosis rate of testicular germ cells in each group was 57.44% in germ cell group, 28.54% in CT group, 30.11% in MTa group and 28.59% in MTb group, the difference was statistically significant (P < 0.01). There was no significant difference in apoptosis rate between MN group and MT group (46.54%) and MT group (43.41%) (P> 0.05). There was significant difference in the percentage of testicular germ cells expressing P53 protein between MN, CT, Qinglv Shengjing Wan group and MTD group (P <0.01). Conclusion Escherichia coli infection in mice, can lead to mouse testes germ cell P53 protein expression increased, germ cell apoptosis increased. Qingloryjing Pill can down-regulate P53 protein, reduce the rate of germ cell apoptosis and improve the reproductive ability of mice. Therefore, the Qing Li Sheng Jing pill is one of the effective drugs for the treatment of Escherichia coli infective infertility.