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目的 :人工角膜支架材料植入角膜板层囊带后 ,观测GAGs在角膜及支架材料中的沉积及合成。方法 :将人工角膜支架材料制成圆盘状以手术方法植入角膜板层囊带内 ,对侧未手术眼及仅制备囊带眼分别为正常对照及手术对照。GAGs经 4M guanidin HCI提取 ,提取物经Q sepharose柱层析。应用选择性多糖酶消化测定GAGs。结果 :人工角膜支架材料植入角膜实质层后 ,角膜总GAG的含量降低 ,而DS CS/KS ,HS/KS比率在周围组织和材料中增加。结论 :将人工角膜支架材料植入角膜实质层 ,引起材料内和周围角膜组织GAGs的组成及硫酸化的改变。术后 84d材料内GAGs的组成与周围角膜组织相似 ,表明支架材料逐渐被周围角膜组织所接受。
OBJECTIVE: To observe the deposition and synthesis of GAGs in corneal and scaffolds after implantation of corneal lamellar capsular strips. Methods: The artificial corneal scaffolds were made into a disc shape and surgically implanted into the lamellar layer of the corneal lamina. The unopposed eyes on the contralateral side and the capsular eye were prepared respectively for normal control and operation control. GAGs were extracted with 4M guanidin HCI and the extracts were chromatographed on a Q sepharose column. GAGs were assayed using selective polysaccharide enzyme digestion. Results: The total corneal total GAG content decreased after the artificial corneal scaffold material was implanted into corneal parenchyma, while the ratio of DS CS / KS and HS / KS increased in surrounding tissues and materials. CONCLUSIONS: The implantation of artificial corneal scaffolds into the parenchyma of cornea causes changes in the composition and sulfation of GAGs in and around the cornea. The composition of GAGs in the material after 84 days was similar to that of the surrounding corneal tissue, indicating that the scaffold material was gradually accepted by the surrounding corneal tissue.