Objective: Discussion on the protectiion of electroacupuncture “Shenmen” on heart and brain injury induced by acute myocardial ischemia in rats from the perspective of the expression of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP). Methods: Thirty male SD rats were randomly divided into normal group, model group, and electroacupuncture group. The electrocardiogram was recorded by the Powerlab 8-lead physiological recording system. The left anterior descending coronary artery was ligated to replicate the rat myocardial ischemia model. The acupuncture group was treated with electroacupuncture on the second day after the model was replicated. After the last electroacupuncture treatment, rat myocardium, hippocampus tissue and abdominal aortic blood were collected, and enzyme-linked immunosorbent assay was used to detect the levels of cAMP and cGMP in myocardium, hippocampus tissue and serum content. Results: Compared with the normal group, the cAMP content in the myocardial tissue of the model group was significantly increased, and the cAMP content in the hippocampus tissue and serum was significantly reduced; compared with the model group, the cAMP content in the myocardial tissue of the electroacupuncture group was decreased, and the hippocampus tissue Compared with the normal group, the content of cGMP in the myocardial tissue and serum of the model group increased, and the content of cGMP in the hippocampus decreased. Compared with the model group, the content of cGMP in the hippocampus of the electroacupuncture group was increased. The cGMP content increased, the serum cGMP content was significantly reduced, and the difference in the cGMP content in myocardial tissue was not statistically significant. Conclusion: Electroacupuncture at “Shenmen” acupoint can significantly improve the expression of cAMP, cGMP and myocardial cAMP in the serum and hippocampus of model rats with acute myocardial ischemia-induced heart and brain injury, but has a lower effect on myocardial cGMP content.